A0781

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A0781

Sigma

 

Aphidicolin from Nigrospora sphaerica

≥98% (TLC), powder

Synonym:APC
CAS Number:38966-21-1
Linear Formula:C20H34O4
Molecular Weight:338.48
Beilstein Registry Number:4689958
MDL number:MFCD00083214
PubChem Substance ID:24890452

Description

CautionEstimated shelf-life is two years. For optimal stability, it should be protected from light and stored desiccated.
Biochem/physiol ActionsAntibiotic which is a potent antiviral and antimitotic agent and also an inhibitor of DNA polymerase.

Properties

assay≥98% (TLC)
formpowder
optical activity[α]27/D +12°, c = 1 in methanol(lit.)
color white
mp227-233 °C(lit.)
solubilityethanol: 1 mg/mL stable at least a week at 4°C.
 DMSO: 10 mg/mL stable at least six weeks at −20°C.
 methanol: 10 mg/mL
 H2O:  insoluble
Gene Informationhuman ... POLA1(5422), POLD1(5424), POLG(5428)
storage temp.2-8°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements22-24
WGK Germany3
RTECSPB9185000

Related Products

Related productZ703753, Cell Cycle Control: Mechanisms and Protocols

References

referenceSpadari, S., et al., Control of cell division by aphidicolin without adverse effects upon resting cells. Arzneimittelforschung 35, 1108-1116, (1985) Abstract
 Yamada, K., and Itoh, R., Involvement of DNA polymerase delta and/or epsilon in joining UV-induced DNA single strand breaks in human fibroblasts. Biochim. Biophys. Acta 1219, 302-306, (1994) Abstract
 Cheng, C.H., and Kuchta, R.D., DNA polymerase epsilon: aphidicolin inhibition and the relationship between polymerase and exonuclease activity. Biochemistry 32, 8568-8574, (1993) Abstract
 Mutomba, M.C., and Wang, C.C., Effects of aphidicolin and hydroxyurea on the cell cycle and differentiation of Trypanosoma brucei bloodstream forms. Mol. Biochem. Parasitol. 80, 89-102, (1996) Abstract
 Kuwakado, K., et al., Augmentation by aphidicolin of 1-β-D-arabinofuranosylcytosine-induced c-jun and NF-kappa B activation in a human myeloid leukemia cell line: correlation with apoptosis. Leuk. Res. 19, 645, (1995) Abstract
 Wang, N.D., et al., Determination of the specificity of aphidicolin-induced breakage of the human 3p14.2 fragile site. Genomics 17, 341-347, (1993) Abstract
 McAllister, B.F., and Greenbaum, I.F., How common are common fragile sites: variation of aphidicolin-induced chromosomal fragile sites in a population of the deer mouse (Peromyscus maniculatus). Hum. Genet. 100, 182-188, (1997) Abstract
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